My PhD research investigates the molecular mechanisms underlying intra- and intergenerational drought stress memory in oats, with the goal of identifying protein-based markers to enhance climate resilience in cereal crops. Building on my expertise in analytical chemistry and bioactive compound characterization, this project applies advanced proteomic, phosphoproteomic, and peptidomic techniques to uncover how post-translational modifications and signalling peptides regulate drought adaptation at the molecular level.
My research aims to characterize how drought stress memory operates within individual oat plants (intra-generational) and is transmitted to their progeny (intergenerational), and to identify molecular signatures that distinguish drought-tolerant from drought-sensitive cultivars.
Two contrasting oat cultivars will be subjected to controlled drought conditions across two consecutive generations. Tissue samples from grain, leaf, and root will be collected across five developmental stages using global discovery proteomics (data-dependent acquisition), targeted quantification approaches (data-independent acquisition and SWATH-MS), phosphoproteomics, and peptidomics. Multivariate and network analyses will integrate these datasets to identify persistent molecular signatures associated with drought memory and tolerance.
We expect this research will reveal the protein-level mechanisms governing drought stress memory in oats and deliver molecular markers with potential application in breeding programmes for developing drought-resilient cultivars suited to Australian rainfed agriculture. The findings will contribute to advancing sustainable crop production under climate variability and support global food security.